Until recently, PCR was the only method for direct isolation of desirable chromosomal segments from complex genomes. Although it is widely used for the analysis of genomes, PCR has one major ...

To reduce the time dedicated to clone screening in the development of cell lines for stable virus production, a protocol merging the procedures of cell cloning and titer screening was implemented: the ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Developing cell lines for use as accurate models for disease has always been a challenging task. In order to replicate phenotypes or enable downstream experimentation, genome engineering of cell lines ...

Here are steps you can take to increase the odds of generating correct clones. PCR amplicons are commonly used for cloning. Primers made from synthetic oligonucleotides often contain a small ...