Understand the physics behind total internal reflection and why light can become completely trapped inside a medium. This video explains the conditions required for total internal reflection, the role ...

In TIRF microscopy, fluorescent molecules are in a sample in an aqueous environment that is near a solid with a high refractive index, usually a glass coverslip. At what is called the critical angle, ...

TIRF is a microscopy technique that is used to image fluorescent molecules, such as green fluorescent protein (GFP) and fluorochromes, in liquids that are adjacent to a solid with a high refractive ...

1. The reflection of light from the surfaces of many kinds of leaves has been measured by means of a direct reading spectrophotometer, the measurements being made normal (that is, at an angle of 90⚬ ...