You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort ...

In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

PCR-based techniques have transformed how scientists analyze transcripts’ translational applications in cancer and neuroscience research.

Sample volumes that are gradually increasing and innovative options for molecular biology experiments are altering the demands on quantitative real-time PCR. Migrating to the multi-well format with ...

With the global overuse of antibiotics, the emergence of multidrug-resistant (MDR) bacteria and "superbugs" has become a major threat to human health and food safety. Among these, drug-resistant ...

Scientists around the world employ quantitative real-time PCR (qPCR) to measure the amount of particular nucleic acid sequences within a sample. While this technique is rapid, sensitive, and ...

Early molecular response in patients (pts) with chronic myeloid leukemia in chronic phase (CML-CP) treated with dasatinib (DAS) or imatinib (IM) from DASISION. Survival outcomes of sustained MR4.5 in ...

A randomized trial of chemoradiation using mitomycin or cisplatin, with or without maintenance cisplatin/5FU in squamous cell carcinoma of the anus (ACT II) This is an ASCO Meeting Abstract from the ...

Quantitative genetic analysis plays a critical role in the research and development of novel biotherapeutics. As a sensitive and precise method for quantitating specific nucleic acid targets, qPCR is ...