Beer's Law: Absorbance & Concentration
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Beer's Law: Absorbance & Concentration
Beer's law expresses the relationship between light intensity and the concentration of a substance through a formula. Learn the background of light transmittance and absorbance, and how the equation of Beer's law is used in science.
23K viewsFeb 12, 2016
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How do you calculate concentration from absorbance?
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SOLVED: What is the relationship between absorbance and protein concentration? Use a graph to explain. What are the advantages and disadvantages of the technique? What are the spectroscopic characteristics of myoglobin? Explain Bradford assays and their importance. How do they work? How do you make a standard curve? What are protein dyes, specifically Coomassie? What do they do and how do they work? Relate it to UV-Vis and how absorption works in general. Describe why we absorb certain wavelengt
SOLVED: What is the relationship between absorbance and protein concentration? Use a graph to explain. What are the advantages and disadvantages of the technique? What are the spectroscopic characteristics of myoglobin? Explain Bradford assays and their importance. How do they work? How do you make a standard curve? What are protein dyes, specifically Coomassie? What do they do and how do they work? Relate it to UV-Vis and how absorption works in general. Describe why we absorb certain wavelengt
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How do you calculate concentration from absorbance?
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SOLVED: What is the relationship between absorbance and protein concentration? Use a graph to explain. What are the advantages and disadvantages of the technique? What are the spectroscopic characteristics of myoglobin? Explain Bradford assays and their importance. How do they work? How do you make a standard curve? What are protein dyes, specifically Coomassie? What do they do and how do they work? Relate it to UV-Vis and how absorption works in general. Describe why we absorb certain wavelengt
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